Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides:comparison of single and mixture effects

Strong, Becky and Halsall, Crispin James and Jones, Kevin Christopher and Shore, Richard Francis and Martin, Francis Luke (2016) Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides:comparison of single and mixture effects. Aquatic Toxicology, 178. pp. 8-18. ISSN 1879-1514

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Abstract

Amphibians are regarded as sensitive sentinels of environmental pollution due to their permeable skin and complex life cycle, which usually involves reproduction and development in the aquatic environment. Fungicides are widely applied agrochemicals and have been associated with developmental defects in amphibians; thus, it is important to determine chronic effects of environmentally-relevant concentrations of such contaminants in target cells. Infrared (IR) spectroscopy has been employed to signature the biological effects of environmental contaminants through extracting key features in IR spectra with chemometric methods. Herein, the Xenopus laevis (A6) cell line was exposed to low concentrations of carbendazim (a benzimidazole fungicide) or flusilazole (a triazole fungicide) either singly or as a binary mixture. Cells were then examined using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy coupled with multivariate analysis. Results indicate significant changes in the IR spectra of cells induced by both agents at all concentrations following single exposures, primarily in regions associated with protein and phospholipids. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.

Item Type:
Journal Article
Journal or Publication Title:
Aquatic Toxicology
Additional Information:
This is the author’s version of a work that was accepted for publication in Aquatic Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, 178, 2016 DOI: 10.1016/j.aquatox.2016.07.005
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2300/2307
Subjects:
ID Code:
80638
Deposited By:
Deposited On:
02 Aug 2016 09:14
Refereed?:
Yes
Published?:
Published
Last Modified:
24 Oct 2020 04:18