Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion

Benz, Corinna and Kovárová, Julie and Králová-Hromadová, Ivica and Pierlik, Antonio J. and Lukeš, Julius (2016) Roles of the Nfu Fe-S targeting factors in the trypanosome mitochondrion. International Journal for Parasitology, 46 (10). pp. 641-651. ISSN 0020-7519

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Iron–sulphur clusters (ISCs) are protein co-factors essential for a wide range of cellular functions. The core iron–sulphur cluster assembly machinery resides in the mitochondrion, yet due to export of an essential precursor from the organelle, it is also needed for cytosolic and nuclear iron–sulphur cluster assembly. In mitochondria all [4Fe–4S] iron–sulphur clusters are synthesised and transferred to specific apoproteins by so-called iron–sulphur cluster targeting factors. One of these factors is the universally present mitochondrial Nfu1, which in humans is required for the proper assembly of a subset of mitochondrial [4Fe–4S] proteins. Although most eukaryotes harbour a single Nfu1, the genomes of Trypanosoma brucei and related flagellates encode three Nfu genes. All three Nfu proteins localise to the mitochondrion in the procyclic form of T. brucei, and TbNfu2 and TbNfu3 are both individually essential for growth in bloodstream and procyclic forms, suggesting highly specific functions for each of these proteins in the trypanosome cell. Moreover, these two proteins are functional in the iron–sulphur cluster assembly in a heterologous system and rescue the growth defect of a yeast deletion mutant.

Item Type:
Journal Article
Journal or Publication Title:
International Journal for Parasitology
Additional Information:
This is the author’s version of a work that was accepted for publication in International Journal for Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal for Parasitology, 46 (10), 2016 DOI: 10.1016/j.ijpara.2016.04.006
Uncontrolled Keywords:
?? trypanosoma bruceinfu1iron–sulphur clusterfe–smitochondrioninfectious diseasesparasitology ??
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Deposited On:
13 Jun 2016 15:18
Last Modified:
14 Jul 2024 01:20