Variants of the elongator protein 3 (ELP3) gene are associated with motor neuron degeneration

Simpson, Claire L. and Lemmens, Robin and Miskiewicz, Katarzyna and Broom, Wendy J. and Hansen, Valerie K. and van Vught, Paul W. J. and Landers, John E. and Sapp, Peter and Van Den Bosch, Ludo and Knight, Joanne and Neale, Benjamin M. and Turner, Martin R. and Veldink, Jan H. and Ophoff, Roel A. and Tripathi, Vineeta B. and Beleza, Ana and Shah, Meera N. and Proitsi, Petroula and Van Hoecke, Annelies and Carmeliet, Peter and Horvitz, H. Robert and Leigh, P. Nigel and Shaw, Christopher E. and van den Berg, Leonard H. and Sham, Pak C. and Powell, John F. and Verstreken, Patrik and Brown, Robert H. and Robberecht, Wim and Al-Chalabi, Ammar (2009) Variants of the elongator protein 3 (ELP3) gene are associated with motor neuron degeneration. Human Molecular Genetics, 18 (3). pp. 472-481. ISSN 0964-6906

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Abstract

Amyotrophic lateral sclerosis (ALS) is a spontaneous, relentlessly progressive motor neuron disease, usually resulting in death from respiratory failure within 3 years. Variation in the genes SOD1 and TARDBP accounts for a small percentage of cases, and other genes have shown association in both candidate gene and genome-wide studies, but the genetic causes remain largely unknown. We have performed two independent parallel studies, both implicating the RNA polymerase II component, ELP3, in axonal biology and neuronal degeneration. In the first, an association study of 1884 microsatellite markers, allelic variants of ELP3 were associated with ALS in three human populations comprising 1483 people (P=1.96 x 10(-9)). In the second, an independent mutagenesis screen in Drosophila for genes important in neuronal communication and survival identified two different loss of function mutations, both in ELP3 (R475K and R456K). Furthermore, knock down of ELP3 protein levels using antisense morpholinos in zebrafish embryos resulted in dose-dependent motor axonal abnormalities [Pearson correlation: -0.49, P=1.83 x 10(-12) (start codon morpholino) and -0.46, P=4.05 x 10(-9) (splice-site morpholino), and in humans, risk-associated ELP3 genotypes correlated with reduced brain ELP3 expression (P=0.01). These findings add to the growing body of evidence implicating the RNA processing pathway in neurodegeneration and suggest a critical role for ELP3 in neuron biology and of ELP3 variants in ALS.

Item Type:
Journal Article
Journal or Publication Title:
Human Molecular Genetics
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1311
Subjects:
?? adultagedaged, 80 and overamyotrophic lateral sclerosisanimalsdrosophilaeuropean continental ancestry groupfemalegenetic predisposition to diseasegenetic variationhistone acetyltransferaseshumansmalemicemice, transgenicmiddle agedmotor neuronsmutationnerv ??
ID Code:
79901
Deposited By:
Deposited On:
07 Jun 2016 15:46
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 16:06