Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy

Sowoidnich, Kay and Churchwell, John and Kerns, Jemma Gillian and Buckley, Kevin and Goodship, Allen and Parker, Anthony W. and Matousek, Pavel (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. Journal of Raman Spectroscopy, 47 (2). pp. 240-247. ISSN 0377-0486

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Abstract

Spatially offset Raman spectroscopy (SORS) is currently being developed as an in vivo tool for bone disease detection, but to date, information about the interrogated volume as influenced by the light propagation and scattering characteristics of the bone matrix is still limited. This paper seeks to develop our general understanding of the sampling depths of SORS in bone specimens as a function of the applied spatial offset. Equine metacarpal bone was selected as a suitable specimen of compact cortical bone large enough to allow several thin slices (600 μm) to be cut from the dorsal surface. Photon migration at 830-nm excitation was studied with five bone slices and a 380 μm-thin polytetrafluoroethylene (PTFE) slice placed consecutively between the layers. To optimize Raman signal recovery of the PTFE with increasing depth within the bone stack required a corresponding increase in spatial offset. For example, to sample effectively at 2.2-mm depth within the bone required an optimal SORS offset of 7mm. However, with a 7-mm offset, the maximum accessible penetration depth from which the PTFE signal could be still recovered was 3.7mm. These results provide essential basic information for developing SORS technology for medical diagnostics in general and optimizing sampling through bone tissue, permitting a better understanding of the relationship between the offset and depth of bone assessed, in particular. Potential applications include the detection of chemically specific markers for changes in bone matrix chemistry localized within the tissue and not present in healthy bone.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Raman Spectroscopy
Additional Information:
This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2500
Subjects:
ID Code:
79768
Deposited By:
Deposited On:
16 Jun 2016 12:44
Refereed?:
Yes
Published?:
Published
Last Modified:
31 Oct 2020 04:14