Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells

Molloy, Jennifer C. and Sommer, Ulf and Viant, Mark R. and Sinkins, Steven Paul (2016) Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells. Applied and Environmental Microbiology, 82 (10). pp. 3109-3120. ISSN 0099-2240

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Abstract

Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia in comparison to uninfected Aa23-T cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia. Most significantly, almost all sphingolipid classes were depleted, and some reductions in diacylglycerols and phosphatidylcholines were also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve our understanding of the intracellular interactions between Wolbachia and mosquitoes. IMPORTANCE Mosquitoes transmit a variety of important viruses to humans, such as dengue virus and Zika virus. Certain strains of the intracellular bacterial genus called Wolbachia found in or introduced into mosquitoes can block the transmission of viruses, including dengue virus, but the mechanisms responsible are not well understood. We found substantial shifts in the cellular lipid profiles in the presence of these bacteria. Some lipid classes previously shown to be enriched in dengue virus-infected mosquito cells were depleted in the presence of Wolbachia, suggesting that Wolbachia may produce a cellular lipid environment that inhibits mosquito-borne viruses.

Item Type:
Journal Article
Journal or Publication Title:
Applied and Environmental Microbiology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1305
Subjects:
ID Code:
79723
Deposited By:
Deposited On:
24 May 2016 09:10
Refereed?:
Yes
Published?:
Published
Last Modified:
09 Sep 2020 03:23