Neuroprotective role of (Val8)GLP-1-Glu-PAL in an in vitro model of Parkinson’s disease

Li, Lin and Liu, Ke and Holscher, Christian and Li, Guanglai and Liu, Yue-Ze (2016) Neuroprotective role of (Val8)GLP-1-Glu-PAL in an in vitro model of Parkinson’s disease. Neural Regeneration Research, 11 (2). pp. 326-331. ISSN 1673-5374

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Abstract

The growth factor glucagon-like peptide-1 (GLP-1) is neuroprotective in several animal models of neurodegeneration. Here, we analyzed the neuroprotective effects of a novel protease-resistant GLP-1 analogue, (Val 8 )GLP-1-Glu-PAL, which has advantages over older analogues, such as improvement of hippocampal neurogenesis, glucose homeostasis, and insulin secretion. We established an in vitro model of Parkinson's disease using the mitochondrial stressor rotenone in primary cultured mouse neurons pretreated with (Val 8 )GLP-1-Glu-PAL. (Val 8 )GLP-1-Glu-PAL alone did not affect neuronal viability, but prevented the rotenone-induced reduction in cell viability in a dose-dependent manner. In addition, (Val 8 )GLP-1-Glu-PAL pretreatment prevented rotenone-induced proapoptotic changes manifesting as downregulation of procaspase-3 and Bcl-2 and upregulation of cleaved caspase-3. These results demonstrate that the novel agent (Val 8 )GLP-1-Glu-PAL shows promise as a drug treatment for Parkinson's disease.

Item Type:
Journal Article
Journal or Publication Title:
Neural Regeneration Research
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2800/2806
Subjects:
ID Code:
79288
Deposited By:
Deposited On:
03 May 2016 13:24
Refereed?:
Yes
Published?:
Published
Last Modified:
28 Oct 2020 04:42