Using Raman spectroscopy to characterize biological materials

Butler, Holly J. and Ashton, Lorna and Bird, Benjamin and Cinque, Gianfelice and Curtis, Kelly and Dorney, Jennifer and Esmonde-White, Karen and Fullwood, Nigel James and Gardner, Benjamin and Martin-Hirsch, Pierre Leonard and Walsh, Michael J. and McAinsh, Martin Robert and Stone, Nicholas and Martin, Francis Luke (2016) Using Raman spectroscopy to characterize biological materials. Nature Protocols, 11 (4). pp. 664-687. ISSN 1754-2189

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Abstract

Raman spectroscopy can be used to measure the chemical composition of a sample, which can in turn be used to extract biological information. Many materials have characteristic Raman spectra, which means that Raman spectroscopy has proven to be an effective analytical approach in geology, semiconductor, materials and polymer science fields. The application of Raman spectroscopy and microscopy within biology is rapidly increasing because it can provide chemical and compositional information, but it does not typically suffer from interference from water molecules. Analysis does not conventionally require extensive sample preparation; biochemical and structural information can usually be obtained without labeling. In this protocol, we aim to standardize and bring together multiple experimental approaches from key leaders in the field for obtaining Raman spectra using a microspectrometer. As examples of the range of biological samples that can be analyzed, we provide instructions for acquiring Raman spectra, maps and images for fresh plant tissue, formalin-fixed and fresh frozen mammalian tissue, fixed cells and biofluids. We explore a robust approach for sample preparation, instrumentation, acquisition parameters and data processing. By using this approach, we expect that a typical Raman experiment can be performed by a nonspecialist user to generate high-quality data for biological materials analysis.

Item Type:
Journal Article
Journal or Publication Title:
Nature Protocols
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300
Subjects:
ID Code:
79129
Deposited By:
Deposited On:
25 Apr 2016 15:42
Refereed?:
Yes
Published?:
Published
Last Modified:
05 Apr 2020 03:56