Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil

Song, Mengke and Jiang, Longfei and Zhang, Dayi and Luo, Chunling and Wang, Yan and Yu, Zhiqiang and Yin, Hua and Zhang, Gan (2016) Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil. Journal of Hazardous Materials, 308. pp. 50-57. ISSN 0304-3894

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Abstract

Information on microorganisms possessing the ability to metabolize different polycyclic aromatic hydrocarbons (PAHs) in complex environments helps in understanding PAHs behavior in natural environment and developing bioremediation strategies. In the present study, stable-isotope probing (SIP) was applied to investigate degraders of PAHs in a forest soil with the addition of individually 13C-labeled phenanthrene, anthracene, and fluoranthene. Three distinct phylotypes were identified as the active phenanthrene-, anthracene- and fluoranthene-degrading bacteria. The putative phenanthrene degraders were classified as belonging to the genus Sphingomona. For anthracene, bacteria of the genus Rhodanobacter were the putative degraders, and in the microcosm amended with fluoranthene, the putative degraders were identified as belonging to the phylum Acidobacteria. Our results from DNA-SIP are the first to directly link Rhodanobacter- and Acidobacteria-related bacteria with anthracene and fluoranthene degradation, respectively. The results also illustrate the specificity and diversity of three- and four-ring PAHs degraders in forest soil, contributes to our understanding on natural PAHs biodegradation processes, and also proves the feasibility and practicality of DNA-based SIP for linking functions with identity especially uncultured microorganisms in complex microbial biota.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Hazardous Materials
Additional Information:
This is the author’s version of a work that was accepted for publication in Journal of Hazardous Materials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hazardous Materials 308, 2016 DOI: 10.1016/j.jhazmat.2016.01.009
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2300/2305
Subjects:
ID Code:
78138
Deposited By:
Deposited On:
08 Feb 2016 10:18
Refereed?:
Yes
Published?:
Published
Last Modified:
27 Sep 2020 02:55