Biochemical characterization of predicted Precambrian RuBisCO

Shih, Patrick M. and Occhialini, Alessandro and Cameron, Jeffrey C. and Andralojc, P. John and Parry, Martin A. J. and Kerfeld, Cheryl A. (2016) Biochemical characterization of predicted Precambrian RuBisCO. Nature Communications, 7. ISSN 2041-1723

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Abstract

The antiquity and global abundance of the enzyme, RuBisCO, attests to the crucial and longstanding role it has played in the biogeochemical cycles of Earth over billions of years. The counterproductive oxygenase activity of RuBisCO has persisted over billions of years of evolution, despite its competition with the carboxylase activity necessary for carbon fixation, yet hypotheses regarding the selective pressures governing RuBisCO evolution have been limited to speculation. Here we report the resurrection and biochemical characterization of ancestral RuBisCOs, dating back to over one billion years ago (Gyr ago). Our findings provide an ancient point of reference revealing divergent evolutionary paths taken by eukaryotic homologues towards improved specificity for CO2, versus the evolutionary emphasis on increased rates of carboxylation observed in bacterial homologues. Consistent with these distinctions, in vivo analysis reveals the propensity of ancestral RuBisCO to be encapsulated into modern-day carboxysomes, bacterial organelles central to the cyanobacterial CO2 concentrating mechanism.

Item Type:
Journal Article
Journal or Publication Title:
Nature Communications
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100
Subjects:
ID Code:
78053
Deposited By:
Deposited On:
29 Jan 2016 09:46
Refereed?:
Yes
Published?:
Published
Last Modified:
22 Sep 2020 02:30