Prenylation of the floral transcription factor APETALA1 modulates its function

Yalovsky, Shaul and Rodríguez-Concepción, Manuel and Bracha, Keren and Toledo-Ortiz, Gabriela and Gruissem, Wilhelm (2000) Prenylation of the floral transcription factor APETALA1 modulates its function. Plant Cell, 12 (8). pp. 1257-1266. ISSN 1040-4651

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Abstract

The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro and in vivo. AP1 regulates the transition from inflorescence shoot to floral meristems and the development of sepals and petals. AP1 fused to green fluorescent protein (GFP) retained transcription factor activity and directed the expected terminal flower phenotype when ectopically expressed in transgenic Arabidopsis. However, ap1mS, a farnesyl cysteine-acceptor mutant of AP1, as well as the GFP-ap1mS fusion protein failed to direct the development of compound terminal flowers but instead induced novel phenotypes when ectopically expressed in Arabidopsis. Similarly, compound terminal flowers did not develop in era1-2 transformants that ectopically expressed AP1. Together, the results demonstrate that AP1 is a target of farnesyltransferase and suggest that farnesylation alters the function and perhaps specificity of the transcription factor.

Item Type: Journal Article
Journal or Publication Title: Plant Cell
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1300/1307
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 77864
Deposited By: ep_importer_pure
Deposited On: 21 Jan 2016 09:26
Refereed?: Yes
Published?: Published
Last Modified: 12 Feb 2020 02:51
URI: https://eprints.lancs.ac.uk/id/eprint/77864

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