Miniaturised isotachophoresis of DNA

Prest, Jeff E. and Baldock, Sara J. and Day, Philip J. R. and Fielden, Peter R. and Goddard, Nicholas J. and Brown, Bernard J. Treves (2007) Miniaturised isotachophoresis of DNA. Journal of Chromatography A, 1156 (1-2). pp. 154-159. ISSN 0021-9673

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Abstract

This paper presents the findings of a feasibility study investigating the behaviour of DNA under conditions of miniaturised isotachophoresis. An electrolyte system comprising a leading electrolyte of 5 mM perchloric acid at pH 6.0 and a terminating electrolyte of 10 mM gallic acid was devised and used to perform isotachophoresis of DNA containing samples on a miniaturised poly(methyl methacrylate) device. Under such conditions it was found that no separation of DNA fragments was observed with the substance migrating instead as a single isotachophoretic zone. Whilst such a result shows the method is unsuitable for analysis DNA it offers significant potential as a means of sample preparation for subsequent analysis using another method. This is because the single zone of DNA formed is preconcentrated to a constant concentration governed by the leading ion and is separated from all species with different effective electrophoretic mobilities.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Chromatography A
Subjects:
ID Code:
77499
Deposited By:
Deposited On:
06 Jan 2016 10:30
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 09:32