Analysis of amino acids by miniaturised isotachophoresis

Prest, Jeff E. and Baldock, Sara J. and Fielden, Peter R. and Goddard, Nicholas J. and Treves Brown, Bernard J. (2004) Analysis of amino acids by miniaturised isotachophoresis. Journal of Chromatography A, 1051 (1-2). pp. 221-226. ISSN 0021-9673

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A method allowing the miniaturised isotachophoretic analysis of amino acids has been developed. To overcome the problems of carbonate contamination which occur when performing separations at alkaline pH levels glycolate was used as the leading ion. Addition of magnesium to the leading electrolyte as a counter species was found to improve the separations. The method has been used on a poly(methyl methacrylate) microdevice with integrated on-column conductivity detectors. The behaviour of a range of common amino acids was investigated and successful separations of up to seven amino acids were made. Good linearity was observed with calibration curves for aspartic acid and phenylalanine over the range 0.063–1.0 mM. Limits of detection for these two species were calculated to be 0.060 and 0.018 mM, respectively.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Chromatography A
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23 Dec 2015 09:08
Last Modified:
15 Jul 2024 15:41