Zhao, Xiaohui and Li, Hanbing and Ding, Aihong and Zhou, Guizhong and Sun, Yujiao and Zhang, Dayi (2016) Preparing and characterizing Fe3O4@cellulose nanocomposites for effective isolation of cellulose-decomposing microorganisms. Materials Letters, 163. pp. 154-157. ISSN 0167-577X
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Abstract
This study developed Fe3O4@cellulose nanocomposites by co-precipitation synthesis for bacteria capture and isolation. By surface modification with cellulose, the Fe3O4@cellulose nanocomposites have 20 nm average particle size and 3.3–24.9 emu/g saturation magnetization. Living bacteria could be captured by the Fe3O4@cellulose nanocomposites and harvested by magnetic field, with high efficiency (95.1%) and stability (>99.99%). By metabolizing cellulose and destroying the Fe3O4@cellulose@bacteria complex, cellulose-decomposing microorganisms lost the magnetism. They were therefore able to be isolated from the inert microbial community and the separation efficiency achieved over 99.2%. This research opened a door to cultivate the uncultivable cellulose-decomposing microorganisms in situ and further characterize their ecological functions in natural environment.