An investigation of ribulosebisphosphate carboxylase activity by high resolution 1H NMR

Gutteridge, Steven and Parry, Martin A J and Schmidt, C. N Godfrey and Feeney, Jim (1984) An investigation of ribulosebisphosphate carboxylase activity by high resolution 1H NMR. FEBS Letters, 170 (2). pp. 355-359. ISSN 0014-5793

Abstract

The reactions catalysed by ribulosebisphosphate carboxylase/oxygenase have been studied by high resolution proton NMR spectroscopy. The protons of the substrate ribulose-P2 and the products of the carboxylase and oxygenase reactions are readily assigned to the various carbon centres. Half of the 3-phosphoglycerate molecules generated during carboxylase turnover of ribulose-P2 in saturating CO2 concentrations acquire deuterium from the solution at the C2 position, whereas the other 3-phosphoglycerate molecules and also the 3-phosphoglycerate produced as a result of the oxygenase activity contain no deuterium of this type. However, the addition of activated enzyme (i.e., in the presence of an effector) to a solution of ribulose-P2 in the near absence of either CO2 or O2 catalyses the exchange of the C3 proton with the deuterium of the solution.