Rapid kinetics of an N-terminal mutant of cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase

Brändén, R. and Keys, A. J. and Parry, M. A J (1990) Rapid kinetics of an N-terminal mutant of cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 1037 (3). pp. 328-331. ISSN 0167-4838

Abstract

The transient changes in absorption of visible light upon addition of ribulose 1,5-bisphosphate to Co2+-activated ribulose-1,5-bisphosphate carboxylase/oxygenase were used to show altered catalytic properties of a mutant form of the enzyme from Anacystis nidulans. The mutant form of the enzyme had a modified N-terminus and a 10-fold greater Km for ribulose 1,5-bisphosphate than the natural cyanobacterial enzyme.