Rapid kinetics of an N-terminal mutant of cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase

Brändén, R. and Keys, A. J. and Parry, M. A J (1990) Rapid kinetics of an N-terminal mutant of cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 1037 (3). pp. 328-331. ISSN 0167-4838

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Abstract

The transient changes in absorption of visible light upon addition of ribulose 1,5-bisphosphate to Co2+-activated ribulose-1,5-bisphosphate carboxylase/oxygenase were used to show altered catalytic properties of a mutant form of the enzyme from Anacystis nidulans. The mutant form of the enzyme had a modified N-terminus and a 10-fold greater Km for ribulose 1,5-bisphosphate than the natural cyanobacterial enzyme.

Item Type:
Journal Article
Journal or Publication Title:
Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1315
Subjects:
ID Code:
76057
Deposited By:
Deposited On:
21 Oct 2015 05:04
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 09:25