Shikanai, Toshiharu and Foyer, Christine H. and Dulieu, Hubert and Parry, Martin A. J. and Yokota, Akiho (1996) A point mutation in the gene encoding the Rubisco large subunit interferes with holoenzyme assembly. Plant Molecular Biology, 31 (2). pp. 399-403. ISSN 0167-4412
Full text not available from this repository.Abstract
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), a key enzyme of photosynthetic CO2 fixation, is composed of 8 large and 8 small subunits. The Rubisco-deficient Nicotiana tabacum mutant Sp25 is able to synthesize the peptides for both subunits but does not contain any active holoenzyme. The phenotype is maternally inherited and thus caused by a mutation in the chloroplast genome, which also encodes the Rubisco large subunit. A comparison of the nucleotide sequences of the large subunit gene of the Sp25 mutant with that of the wild-type tobacco revealed a single nucleotide change in the Sp25 mutant. This resulted in an amino acid substitution at Gly-322, which was replaced by serine.