Altered Rubisco activity and amounts of a daytime tight-binding inhibitor in transgenic tobacco expressing limiting amounts of phosphoribulokinase

Paul, Matthew J. and Andralojc, P. John and Banks, Fiona M. and Parry, Martin A J and Knight, Jacqui S. and Gray, John C. and Keys, Alfred J. (1996) Altered Rubisco activity and amounts of a daytime tight-binding inhibitor in transgenic tobacco expressing limiting amounts of phosphoribulokinase. Journal of Experimental Botany, 47 (12). pp. 1963-1966. ISSN 0022-0957

Abstract

Transgenic tobacco with RuBP-limited photosynthetic assimilation due to a 95% reduction in phosphoribulokinase activity, had higher specific activities of Rubisco in fresh extracts and after full activation, than in the wild type. Differences in the amounts of a daytime tight-binding inhibitor were sufficient to contribute significantly to these activity differences.