Separation of the branch chain hexose, hamamelose, by high-performance liquid chromatography, and amperometric detection of hamamelose and related compounds

Andralojc, P. John and Keys, Alfred J. and Adam, Alex and Parry, M. A J (1998) Separation of the branch chain hexose, hamamelose, by high-performance liquid chromatography, and amperometric detection of hamamelose and related compounds. Journal of Chromatography A, 814 (1-2). pp. 105-110. ISSN 0021-9673

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Abstract

The identification and purification of the branch chain hexose hamamelose (2-hydroxymethyl-d-ribose) from plant leaves is described. The procedure includes metabolite extraction in acid and a series of isocratic HPLC separations. Amperometric detection of hamamelose is shown to be quantitative, having a detection limit below 1 nmol. Similar detection of the related compound, 2-carboxy-d-arabinitol, and its monophosphate ester, yields unusually small peaks. Copyright (C) 1998 Elsevier Science B.V.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Chromatography A
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1600/1602
Subjects:
?? carboxyarabinitolenzymeshamameloseplant materialsrubiscosugarsanalytical chemistry ??
ID Code:
76034
Deposited By:
Deposited On:
21 Oct 2015 05:04
Refereed?:
Yes
Published?:
Published
Last Modified:
18 Sep 2024 15:15