Arabidopsis sucrose non-fermenting-1-related protein kinase-1 and calcium-dependent protein kinase phosphorylate conserved target sites in ABA response element binding proteins

Zhang, Y. and Andralojc, P. J. and Hey, S. J. and Primavesi, L. F. and Specht, M. and Koehler, J. and Parry, M. A J and Halford, N. G. (2008) Arabidopsis sucrose non-fermenting-1-related protein kinase-1 and calcium-dependent protein kinase phosphorylate conserved target sites in ABA response element binding proteins. Annals of Applied Biology, 153 (3). pp. 401-409. ISSN 0003-4746

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Abstract

Evidence is provided that plant transcription factors of the ABA response element binding protein (AREBP) class are phosphorylated by sucrose non-fermenting-1-related protein kinase-1 (SnRK1) and a calcium-dependent protein kinase at highly conserved target sites. Two target sites for SnRK1 were identified in AREBPs using a specially developed motif search pipeline. Peptides containing the AREBP sites were phosphorylated by purified SnRK1 in vitro and by calcium-dependent and calcium-independent activities present in soluble protein extracted from Arabidopsis seedlings grown in liquid culture. Most (69-77%) of the calcium-independent phosphorylation of these peptides was removed by immunoprecipitation using anti-SnRK1 antisera, linking this activity unambiguously to SnRK1. It is possible that the protein kinase responsible for the calcium-dependent activity was SnRK3, a protein kinase that is related to SnRK1 and which has similar requirements for target site recognition. However, the involvement of other calcium-dependent protein kinases cannot be ruled out.

Item Type:
Journal Article
Journal or Publication Title:
Annals of Applied Biology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1100/1102
Subjects:
ID Code:
75995
Deposited By:
Deposited On:
21 Oct 2015 05:03
Refereed?:
Yes
Published?:
Published
Last Modified:
13 May 2020 03:05