Purification of Rubisco activase from leaves or after expression in Escherichia coli

Barta, Csengele and Carmo-Silva, A. Elizabete and Salvucci, Michael E. (2011) Purification of Rubisco activase from leaves or after expression in Escherichia coli. Methods in Molecular Biology, 684. pp. 363-374. ISSN 1064-3745

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Abstract

Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco activase from leaves and bacterial cells, respectively.

Item Type:
Journal Article
Journal or Publication Title:
Methods in Molecular Biology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1312
Subjects:
?? MEDICINE(ALL)GENETICSMOLECULAR BIOLOGY ??
ID Code:
75867
Deposited By:
Deposited On:
21 Oct 2015 05:01
Refereed?:
Yes
Published?:
Published
Last Modified:
17 Sep 2023 01:42