Purification of Rubisco activase from leaves or after expression in Escherichia coli

Barta, Csengele and Carmo-Silva, A. Elizabete and Salvucci, Michael E. (2011) Purification of Rubisco activase from leaves or after expression in Escherichia coli. Methods in Molecular Biology, 684. pp. 363-374. ISSN 1064-3745

Abstract

Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco activase from leaves and bacterial cells, respectively.