Rubisco activase activity assays

Barta, Csengele and Carmo-Silva, A. Elizabete and Salvucci, Michael E. (2011) Rubisco activase activity assays. Methods in Molecular Biology, 684. pp. 375-382. ISSN 1064-3745

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Abstract

Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites, thereby restoring the sites to catalytic competence. This chapter reports methods developed for assaying the two activities of Rubisco activase: ATP hydrolysis and Rubisco activation.

Item Type: Journal Article
Journal or Publication Title: Methods in Molecular Biology
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1300/1312
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 75866
Deposited By: ep_importer_pure
Deposited On: 21 Oct 2015 05:01
Refereed?: Yes
Published?: Published
Last Modified: 15 Jan 2020 07:53
URI: https://eprints.lancs.ac.uk/id/eprint/75866

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