Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves.

Carmo-Silva, A. Elizabete and Barta, Csengele and Salvucci, Michael E. (2010) Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves. Methods in Molecular Biology, 684. pp. 339-347. ISSN 1064-3745

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Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a multifunctional enzyme that catalyzes the fixation of CO2 and O2 in photosynthesis and photorespiration, respectively. As the rate-limiting step in photosynthesis, improving the catalytic properties of Rubisco has long been viewed as a viable strategy for increasing plant productivity. Advances in biotechnology have made this goal more attainable by making it possible to modify Rubisco in planta. To properly evaluate the properties of Rubisco, it is necessary to isolate the enzyme in pure form. This chapter describes procedures for rapid and efficient purification of Rubisco from leaves of several species.

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Journal Article
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Methods in Molecular Biology
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21 Oct 2015 05:01
Last Modified:
22 Nov 2022 02:19