Rubisco activity is associated with photosynthetic thermotolerance in a wild rice (Oryza meridionalis)

Scafaro, Andrew P. and Yamori, Wataru and Carmo-Silva, A. Elizabete and Salvucci, Michael E. and von Caemmerer, Susanne and Atwell, Brian J. (2012) Rubisco activity is associated with photosynthetic thermotolerance in a wild rice (Oryza meridionalis). Physiologia Plantarum, 146 (1). pp. 99-109. ISSN 0031-9317

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Abstract

Oryza meridionalis is a wild species of rice, endemic to tropical Australia. It shares a significant genome homology with the common domesticated rice Oryza sativa. Exploiting the fact that the two species are highly related but O. meridionalis has superior heat tolerance, experiments were undertaken to identify the impact of temperature on key events in photosynthesis. At an ambient CO2 partial pressure of 38 Pa and irradiance of 1500 μmol quanta m-2 s-1, the temperature optimum of photosynthesis was 33.7 ± 0.8°C for O. meridionalis, significantly higher than the 30.6 ± 0.7°C temperature optimum of O. sativa. To understand the basis for this difference, we measured gas exchange and rubisco activation state between 20 and 42°C and modeled the response to determine the rate-limiting steps of photosynthesis. The temperature response of light respiration (Rlight) and the CO2 compensation point in the absence of respiration (Γ*) were determined and found to be similar for the two species. C3 photosynthesis modeling showed that despite the difference in susceptibility to high temperature, both species had a similar temperature-dependent limitation to photosynthesis. Both rice species were limited by ribulose-1,5-bisphosphate (RuBP) regeneration at temperatures of 25 and 30°C but became RuBP carboxylation limited at 35 and 40°C. The activation state of rubisco in O. meridionalis was more stable at higher temperatures, explaining its greater heat tolerance compared with O. sativa.

Item Type:
Journal Article
Journal or Publication Title:
Physiologia Plantarum
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1314
Subjects:
ID Code:
75862
Deposited By:
Deposited On:
21 Oct 2015 05:01
Refereed?:
Yes
Published?:
Published
Last Modified:
07 Apr 2020 03:26