Novel phenanthrene-degrading bacteria identified by DNA-stable isotope probing

Jiang, Longfei and Song, Mengke and Luo, Chunling and Zhang, Dayi and Zhang, Gan (2015) Novel phenanthrene-degrading bacteria identified by DNA-stable isotope probing. PLoS ONE, 10 (6). ISSN 1932-6203

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Abstract

Microorganisms responsible for the degradation of phenanthrene in a clean forest soil sample were identified by DNA-based stable isotope probing (SIP). The soil was artificially amended with either <sup>12</sup>C- or <sup>13</sup>C-labeled phenanthrene, and soil DNA was extracted on days 3, 6 and 9. Terminal restriction fragment length polymorphism (TRFLP) results revealed that the fragments of 219- And 241-bp in HaeIII digests were distributed throughout the gradient profile at three different sampling time points, and both fragments were more dominant in the heavy fractions of the samples exposed to the <sup>13</sup>C-labeled contaminant. 16S rRNA sequencing of the <sup>13</sup>C-enriched fraction suggested that Acidobacterium spp. within the class Acidobacteria, and Collimonas spp. within the class Betaproteobacteria, were directly involved in the uptake and degradation of phenanthrene at different times. To our knowledge, this is the first report that the genus Collimonas has the ability to degrade PAHs. Two PAH-RHD<inf>α</inf> genes were identified in <sup>13</sup>C-labeled DNA. However, isolation of pure cultures indicated that strains of Staphylococcus sp. PHE-3, Pseudomonas sp. PHE- 1, and Pseudomonas sp. PHE-2 in the soil had high phenanthrene-degrading ability. This emphasizes the role of a culture-independent method in the functional understanding of microbial communities in situ.

Item Type:
Journal Article
Journal or Publication Title:
PLoS ONE
Additional Information:
Date of Acceptance: 13/05/2015 Publisher version © 2015 Jiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700
Subjects:
ID Code:
75677
Deposited By:
Deposited On:
28 Sep 2015 11:05
Refereed?:
Yes
Published?:
Published
Last Modified:
27 Sep 2020 02:40