Towards a simplified model membrane of skin lipids:preparation and characterisation of a ternary lipid mixture

Ghonaim, Hassan M. and Periasamy, Nagarajan and Noro, Massimo G. and Anwar, Jamshed (2014) Towards a simplified model membrane of skin lipids:preparation and characterisation of a ternary lipid mixture. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (2). pp. 148-152. ISSN 0975-1491

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Abstract

Objective: To develop a simple but a robust model membrane comprising the key generic components of the skin lipid matrix. Methods: We prepared a model membrane comprising an equimolar mixture of ceramide [NS], palmitic acid, and cholesterol. The membrane was deposited on a solid porous substrate (50 nm porous polycarbonate filter) as a continuous lamellar layer. The membrane’s biophysical properties were characterized and the permeability of the membrane to both benzoic acid and caffeine evaluated. Results: The deposited membrane’s biophysical properties were characterized using a variety of methods including Laurdan fluorescence spectroscopy, SEM, and Raman scattering. The permeability co-efficients (kp) of the model membrane to benzoic acid and the hydrophilic molecule caffeine were determined to be of the same order of magnitude as that through real stratum corneum. Conclusions: A simple but robust model membrane for skin lipids has been developed that can be deposited to give a continuous coverage on a porous substrate. It’s permeability to caffeine and benzoic acid has been found to be of the same order as that of real stratum corneum. The model membrane will be invaluable for fundamental mechanistic studies (e.g. effect of penetration enhancers) that can be linked with molecular simulations.

Item Type: Journal Article
Journal or Publication Title: International Journal of Pharmacy and Pharmaceutical Sciences
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/3000/3003
Subjects:
Departments: Faculty of Science and Technology
ID Code: 73840
Deposited By: ep_importer_pure
Deposited On: 18 Jun 2015 05:46
Refereed?: Yes
Published?: Published
Last Modified: 01 Jan 2020 09:13
URI: https://eprints.lancs.ac.uk/id/eprint/73840

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