Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology

Hayes, Polly and Varga, Vladimir and Olego-Fernandez, Sofia and Sunter, Jack and Ginger, Michael and Gull, Keith (2014) Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology. Journal of Cell Biology, 206 (3). pp. 377-384. ISSN 1540-8140

[img]
Preview
PDF (J Cell Biol-2014-Hayes-377-84)
J_Cell_Biol_2014_Hayes_377_84.pdf - Published Version
Available under License Creative Commons Attribution-NonCommercial-ShareAlike.

Download (1MB)

Abstract

Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Cell Biology
Additional Information:
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1307
Subjects:
ID Code:
73714
Deposited By:
Deposited On:
18 Jun 2015 05:42
Refereed?:
Yes
Published?:
Published
Last Modified:
08 Aug 2020 04:16