Interactions of multiwalled carbon nanotubes with algal cells:quantification of association, visualization of uptake, and measurement of alterations in the composition of cells

Rhiem, Stefan and Riding, Matthew J. and Baumgartner, Werner and Martin, Francis L. and Semple, Kirk T. and Jones, Kevin C. and Schäffer, Andreas and Maes, Hanna M. (2015) Interactions of multiwalled carbon nanotubes with algal cells:quantification of association, visualization of uptake, and measurement of alterations in the composition of cells. Environmental Pollution, 196. pp. 431-439. ISSN 0269-7491

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Abstract

Carbon nanotubes (CNTs) are considered promising materials in nanotechnology. We quantified CNT accumulation by the alga Desmodesmus subspicatus. Cells were exposed to radiolabeled CNTs ((14)C-CNTs; 1 mg/L) to determine uptake and association, as well as elimination and dissociation in clear media. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to detect effects of CNTs on algae. CNT-cell interactions were visualized by electron microscopy and related to alterations in their cell composition. A concentration factor of 5000 L/kg dry weight was calculated. Most of the material agglomerated around the cells, but single tubes were detected in the cytoplasm. Computational analyses of the ATR-FTIR data showed that CNT treated algae differed from controls at all sampling times. CNT exposure changed the biochemical composition of cells. The fact that CNTs are bioavailable for algae and that they influence the cell composition is important with regard to environmental risk assessment of this nanomaterial.

Item Type:
Journal Article
Journal or Publication Title:
Environmental Pollution
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3000/3005
Subjects:
ID Code:
72406
Deposited By:
Deposited On:
07 Jan 2015 19:50
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Nov 2020 05:51