Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus : characterization of an anion-selective efflux channel

Roberts, Stephen K. and Dixon, Graham K. and Dunbar, Stuart J. and Sanders, Dale (1997) Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus : characterization of an anion-selective efflux channel. New Phytologist, 137 (4). pp. 579-585. ISSN 0028-646X

Abstract

We have used a pulsed u.v. laser to ablate the cell wall of the filamentous fungus Aspergillus niger van Tiegh., and expose the plasma membrane at defined points along the hypha. Using the patch clamp technique, giga ohm seals (up to 20 G Omega) and recordings of plasma membrane ion channels were obtained. We describe, for the first time in fungi, a 43 pS plasma membrane anion efflux channel. Its potential role in cell signalling and pH homeostasis is discussed.