Wilson, Andrew J. and Ault, James R. and Filby, Maria H. and Philips, Hazel I. A. and Ashcroft, Alison E. and Fletcher, Nicholas C. (2013) Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics. Organic and Biomolecular Chemistry, 11 (13). pp. 2206-2212. ISSN 1477-0520
Full text not available from this repository.Abstract
Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 degrees C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.