Metabolic signatures of human Alzheimer's disease (AD):H-1 NMR analysis of the polar metabolome of post-mortem brain tissue

Graham, Stewart F. and Holscher, Christian and Green, Brian D. (2014) Metabolic signatures of human Alzheimer's disease (AD):H-1 NMR analysis of the polar metabolome of post-mortem brain tissue. Metabolomics, 10 (4). pp. 744-753. ISSN 1573-3882

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Abstract

Brain tissue from so-called Alzheimer's disease (AD) mouse models has previously been examined using H-1 NMR-metabolomics, but comparable information concerning human AD is negligible. Since no animal model recapitulates all the features of human AD we undertook the first H-1 NMR-metabolomics investigation of human AD brain tissue. Human post-mortem tissue from 15 AD subjects and 15 age-matched controls was prepared for analysis through a series of lyophilised, milling, extraction and randomisation steps and samples were analysed using H-1 NMR. Using partial least squares discriminant analysis, a model was built using data obtained from brain extracts. Analysis of brain extracts led to the elucidation of 24 metabolites. Significant elevations in brain alanine (15.4 %) and taurine (18.9 %) were observed in AD patients (p

Item Type:
Journal Article
Journal or Publication Title:
Metabolomics
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1308
Subjects:
ID Code:
71239
Deposited By:
Deposited On:
14 Oct 2014 10:37
Refereed?:
Yes
Published?:
Published
Last Modified:
25 Feb 2020 07:18