Graham, Stewart F. and Holscher, Christian and Green, Brian D. (2014) Metabolic signatures of human Alzheimer's disease (AD):H-1 NMR analysis of the polar metabolome of post-mortem brain tissue. Metabolomics, 10 (4). pp. 744-753. ISSN 1573-3882
Full text not available from this repository.Abstract
Brain tissue from so-called Alzheimer's disease (AD) mouse models has previously been examined using H-1 NMR-metabolomics, but comparable information concerning human AD is negligible. Since no animal model recapitulates all the features of human AD we undertook the first H-1 NMR-metabolomics investigation of human AD brain tissue. Human post-mortem tissue from 15 AD subjects and 15 age-matched controls was prepared for analysis through a series of lyophilised, milling, extraction and randomisation steps and samples were analysed using H-1 NMR. Using partial least squares discriminant analysis, a model was built using data obtained from brain extracts. Analysis of brain extracts led to the elucidation of 24 metabolites. Significant elevations in brain alanine (15.4 %) and taurine (18.9 %) were observed in AD patients (p