The role of the phospholipase C/inositol 1,4,5-trisphosphate-mediated calcium-mobilizing pathway in guard cell signal transduction

McAinsh, Martin and Hetherington, Alistair and Leckie, Callum P. and Mills, Lewis and Ng, Carl K-Y. and Aitken, F. L. and Gray, Julie E. and Hunt, Lee (2000) The role of the phospholipase C/inositol 1,4,5-trisphosphate-mediated calcium-mobilizing pathway in guard cell signal transduction. In: Plant Biology 2000, 2000-07-152000-07-19.

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Abstract

Inositol 1,4,5-trisphosphate (InsP3) is an important component of calcium-based signal transduction pathways in eukaryotic cells. Hydrolysis of phosphatidylinositol 4,5-bisphosphate by phosphoinositide-specific phospholipase C (PI-PLC) generates InsP3, which mobilizes calcium from endomembrane stores, and diacylglycerol. We have used a combined molecular and cell physiological approach to examined the role of the PI-PLC/InsP3-mediated calcium mobilizing pathway in the signal transduction pathway(s) by which stomatal guard cells respond to environmental stimuli such as abscisic acid (ABA) and drought. We have identified a guard cell PI-PLC that is specifically inhibited by an aminosteroid, U-73122; ABA-induced stomatal closure and ABA-induced oscillations in guard cell cytosolic free calcium are both inhibited by U-73122 (Staxen et al., 1999, Proc. Natl. Acad. Sci. USA 96 1779-1784). In addition, microinjection of the competitive InsP3 inhibitor heparin directly into the cytosol of guard cells also inhibits the ABA-induced reduction in guard cell turgor. These data, together with the results of our studies using Nicotiana rustica plants transformed with sense and antisense constructs of the PI-PLC, suggest a role for the PI-PLC/InsP3-mediated calcium-mobilizing pathway in the response of stomata to ABA and drought.

Item Type:
Contribution to Conference (Other)
Journal or Publication Title:
Plant Biology 2000
ID Code:
70280
Deposited By:
Deposited On:
11 Aug 2014 08:54
Refereed?:
No
Published?:
Published
Last Modified:
10 Apr 2020 23:36