High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics

Güther, Maria Lucia S. and Urbaniak, Michael D. and Tavendale, Amy and Prescott, Alan and Ferguson, Michael A. J. (2014) High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics. Journal of Proteome Research, 13 (6). pp. 2796-2806. ISSN 1535-3893

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Abstract

The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed.

Item Type: Journal Article
Journal or Publication Title: Journal of Proteome Research
Additional Information: ACS AuthorChoice/Editors’ Choice via Creative Commons CC-BY Usage Agreement
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1600
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 69491
Deposited By: ep_importer_pure
Deposited On: 27 May 2014 08:29
Refereed?: Yes
Published?: Published
Last Modified: 24 Feb 2020 01:54
URI: https://eprints.lancs.ac.uk/id/eprint/69491

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