Characterisation of DNA methylation status using spectroscopy (mid-IR versus Raman) with multivariate analysis

Kelly, Jemma G. and Najand, Ghazal M. and Martin, Francis L. (2011) Characterisation of DNA methylation status using spectroscopy (mid-IR versus Raman) with multivariate analysis. Journal of Biophotonics, 4 (5). pp. 345-354. ISSN 1864-063X

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Abstract

Methylation status plays important roles in the regulation of gene expression and significantly influences the dynamics, bending and flexibility of DNA. The aim of this study was to determine whether attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman spectroscopy with subsequent multivariate analysis could determine methylation patterning in oligonucleotides variously containing 5-methylcytosine, cytosine and guanine bases. Applied to Low-E reflective glass slides, 10 independent spectral acquisitions were acquired per oligonucleotide sample. Resultant spectra were baseline-corrected and vector normalised over the 1750 cm(-1) -760 cm(-1) (for ATR-FTIR spectroscopy) or the 1750 cm(-1) -600 cm(-1) (for Raman spectroscopy) regions. Data were then analysed using principal component analysis (PCA) coupled with linear discriminant analysis (LDA). Exploiting this approach, biomolecular signatures enabling sensitive and specific discrimination of methylation patterning were derived. For DNA sequence and methylation analysis, this approach has the potential to be an important tool, especially when material is scarce.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Biophotonics
Additional Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2200
Subjects:
ID Code:
69432
Deposited By:
Deposited On:
15 May 2014 11:01
Refereed?:
Yes
Published?:
Published
Last Modified:
29 Jan 2020 07:42