Hepcidin treatment modulates the expression of divalent metal transporter-1, ceruloplasmin, and ferroportin-1 in the rat cerebral cortex and hippocampus

Li, Lin and Hölscher, Christian and Chen, Bing-Bing and Zhang, Zhi-Feng and Liu, Yue-Ze (2011) Hepcidin treatment modulates the expression of divalent metal transporter-1, ceruloplasmin, and ferroportin-1 in the rat cerebral cortex and hippocampus. Biological Trace Element Research, 143 (3). pp. 1581-1593. ISSN 1559-0720

Full text not available from this repository.

Abstract

Elevated iron levels are considered to play a role in the neurodegenerative mechanisms that underlie Alzheimer's and Parkinson's disease. The linkage between hepcidin (Hepc) and ferroportin-1 (FPN1), the divalent metal transporter 1 (DMT1), and ceruloplasmin (CP) in the brain is unknown. To discern the role of Hepc in regulating the expression of these proteins, we investigated FPN1, DMT1, and CP protein and mRNA expression in the brain after the intracerebroventricular injection of Hepc. Our results show that after Hepc injection, expression of FPN1 mRNA and FPN1 protein was inhibited in the cerebral cortex and hippocampus. Furthermore, we showed a clear change of DMT1 and CP protein and mRNA levels in the brain. The immunohistochemical analysis revealed an increase of DMT1 and a decrease of CP levels. Semi-quantitative analysis using PCR methods showed an increase of DMT1(+IRE) mRNA, and a decrease of DMT1(-IRE) mRNA and CP mRNA levels. Since alterations in iron levels in the brain are causally linked to degenerative conditions such as Alzheimer's disease, an improved understanding of the regulation of iron transport protein expression such as FPN1, DMT1, and CP could lead to novel strategies for treatments.

Item Type:
Journal Article
Journal or Publication Title:
Biological Trace Element Research
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1303
Subjects:
?? animalsantimicrobial cationic peptidesbase sequencecation transport proteinscerebral cortexceruloplasmindna primershepcidinshippocampusimmunohistochemistrymalepolymerase chain reactionrna, messengerratsrats, wistarbiochemistryendocrinology, diabetes and m ??
ID Code:
69062
Deposited By:
Deposited On:
04 Apr 2014 11:01
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 14:34