Enhanced stabilization of collagen-based dermal regeneration scaffolds through the combination of physical and chemical crosslinking

Wessels, Quenton and Pretorius, E. (2008) Enhanced stabilization of collagen-based dermal regeneration scaffolds through the combination of physical and chemical crosslinking. South African Journal of Science, 104 (11-12). pp. 513-516. ISSN 0038-2353

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Abstract

The use of collagen in the biomedical device industry has led to major advances in soft tissue repair. This is attributed largely to the favourable biological and physiochemical properties of collagen. Regenerative medicine and tissue engineering favoured the use of this biomaterial and various commercial products have become available in the past few decades. This study aims to develop a collagen and chondroitin-6-sulphate dermal regeneration scaffold with enhanced resistance against enzymatic degradation. Frozen slurries (0.5% collagen) were dried under vacuum, coated with silicone, crosslinked and then thoroughly rinsed. The scaffolds were subjected to a range of quantitative and qualitative tests that included: scanning electron microscopy analysis, collagenase enzymatic degradation, and cytotoxicity assessment. Scaffold resistance to enzymatic degradation was manipulated after dehydrothermal treatment by employing combinations of crosslinking agents, such as glutaraldehyde and / or carbodiimide, with or without the presence of L-lysine. Results indicate that highly porous (mean pore diameter of 87.3 µm), bioactive, non-cytotoxic tissue engineering matrices were obtained. Enhanced stability of these scaffolds was achieved through extensive crosslinking and suggests the potential to prevent in vivo wound contraction sufficiently.

Item Type:
Journal Article
Journal or Publication Title:
South African Journal of Science
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1900
Subjects:
ID Code:
68744
Deposited By:
Deposited On:
28 Feb 2014 11:26
Refereed?:
Yes
Published?:
Published
Last Modified:
24 Mar 2020 03:49