Biocide sensitivity of Vermamoeba vermiformis isolated from dental-unit-waterline systems

Dillon, Mandy and Achilles-Day, Undine and Singhrao, Sim and Pearce, Mark and Morton, L.H. Glyn and Crean, StJohn (2014) Biocide sensitivity of Vermamoeba vermiformis isolated from dental-unit-waterline systems. International Biodeterioration and Biodegradation, 88. pp. 97-105. ISSN 0964-8305

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This study isolated amoebae from two different dental-unit-waterline (DUWL)sources, a simulated laboratory system (sDUWL) and a decommissioned system(dDUWL), within 24 h of it being dismantled from a working clinical practice. Molecular profiles of the isolates were determined and morphological characteristics of the test organisms were examined using microscopy. DNA barcoding identified the amoebae from both sources as being Vermamoeba vermiformis (previously described as Hartmannella vermiformis). These amoebae have been deposited at the Culture Collection of Algae and Protozoa and as CCAP 1534/16, GenBank accession number KC161965 (in-vitro simulated system) and CCAP 1534/17, GenBank accession number KC188996 (decommissioned system). The organisms from both sources exhibited two main forms: motile trophozoites and non-motile cysts. Mature cysts displayed natural fluorescence with excitation and emission wavelength of 488 nm, attributed to the presence of natural flavins and nicotinamide compounds. Both the encysted and the trophozoite forms of V. vermiformis remained unaffected when exposed to the proprietary biocides tested at the concentrations recommended for use in dental surgeries and cysts successfully excysted, to release trophozoites upon subsequent culture. However, two out of three proprietary dental biocides tested on isolated heterotrophic bacteria were effective at controlling bacterial contamination.

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Journal Article
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International Biodeterioration and Biodegradation
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24 Jan 2014 05:53
Last Modified:
22 Nov 2022 00:38