Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination

Chen, Cheng and Zhang, Dayi and Thornton, Steven F. and Duan, Musen and Luo, Yanjun and Ding, Aizhong and Huang, Wei E. (2013) Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination. Environmental Engineering and Management Journal, 12 (7). pp. 1417-1422. ISSN 1582-9596

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Abstract

The detection of environmental pollutants has challenged conventional chemical analysis for the rapid estimation of the bioavailability and toxicity of the contamination impact on human health and ecosystems. Magnetic nanoparticles (MNPs) functionalization as bioreporters can enhance the ability for biosensing in complex contaminated samples such as soils, and the immobilization of bioreporters will promote the detection of gas and online monitoring. In this study, Acinetobacter baylyi ADP1 bioreporters ADPWHlux and ADPWHrecA were used to evaluate the optimization of MNPs bioreporter functionalization and filter immobilization. It demonstrated that the MNPs functionalized and filter-immobilized bioreporters retained a similar sensitivity to the original non-treated bioreporters. These approaches will provide new ways for the detection of contaminants in soil and air samples, with prospects for in situ and online measurement. This study also suggested that portable application and miniaturization is possible when the functionalized bioreporter is immobilized on a filter or magnetic probe.

Item Type: Journal Article
Journal or Publication Title: Environmental Engineering and Management Journal
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2300/2308
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 67947
Deposited By: ep_importer_pure
Deposited On: 10 Dec 2013 14:25
Refereed?: Yes
Published?: Published
Last Modified: 11 Feb 2020 08:18
URI: https://eprints.lancs.ac.uk/id/eprint/67947

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