A captured viral interleukin 10 gene with cellular exon structure

Jayawardane, Gamini and Russell, George C. and Thomson, Jackie and Deane, David and Cox, Helen and Gatherer, Derek and Ackermann, Mathias and Haig, David M. and Stewart, James P. (2008) A captured viral interleukin 10 gene with cellular exon structure. Journal of General Virology, 89 (10). pp. 2447-2455. ISSN 0022-1317

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Abstract

We have characterized a novel, captured and fully functional viral interleukin (IL)-10 homologue ((OvHV)IL-10) from the gammaherpesvirus ovine herpesvirus 2. Unlike IL-10 homologues from other gammaherpesviruses, the (OvHV)IL-10 peptide sequence was highly divergent from that of the host species. The (OvHV)IL-10 gene is unique amongst virus captured genes in that it has precisely retained the original cellular exon structure, having five exons of similar sizes to the cellular counterparts. However, the sizes of the introns are dramatically reduced. The (OvHV)IL-10 protein was shown to be a non-glycosylated, secreted protein of M(r) 21 000 with a signal peptidase cleavage site between amino acids 26 and 27 of the nascent peptide. Functional assays showed that (OvHV)IL-10, in a similar way to ovine IL-10, stimulated mast cell proliferation and inhibited macrophage inflammatory chemokine production. This is the first example of a captured herpesvirus gene retaining the full cellular gene structure.

Item Type:
Journal Article
Journal or Publication Title:
Journal of General Virology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2406
Subjects:
ID Code:
66884
Deposited By:
Deposited On:
30 Sep 2013 23:07
Refereed?:
Yes
Published?:
Published
Last Modified:
20 May 2020 03:02