High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169

Bradley, Amanda J. and Lurain, Nell S. and Ghazal, Peter and Trivedi, Urmi and Cunningham, Charles and Baluchova, Katarina and Gatherer, Derek and Wilkinson, Gavin W. G. and Dargan, Derrick J. and Davison, Andrew J. (2009) High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169. Journal of General Virology, 90 (10). pp. 2375-2380. ISSN 0022-1317

Full text not available from this repository.

Abstract

The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.

Item Type: Journal Article
Journal or Publication Title: Journal of General Virology
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2400/2406
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 66883
Deposited By: ep_importer_pure
Deposited On: 30 Sep 2013 15:07
Refereed?: Yes
Published?: Published
Last Modified: 15 Jan 2020 07:41
URI: https://eprints.lancs.ac.uk/id/eprint/66883

Actions (login required)

View Item View Item