Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore

Urbaniak, Michael D. and Muskett, Frederick W. and Finucane, Michael D. and Caddick, Stephen and Woolfson, Derek N. (2002) Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore. Biochemistry, 41 (39). pp. 11731-11739. ISSN 0006-2960

Full text not available from this repository.

Abstract

The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.

Item Type:
Journal Article
Journal or Publication Title:
Biochemistry
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1303
Subjects:
ID Code:
66402
Deposited By:
Deposited On:
17 Sep 2013 08:09
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 08:37