Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei

Urbaniak, Michael D. (2009) Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei. Molecular and Biochemical Parasitology, 166 (2). pp. 183-185. ISSN 0166-6851

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Abstract

Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.

Item Type:
Journal Article
Journal or Publication Title:
Molecular and Biochemical Parasitology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2405
Subjects:
ID Code:
66390
Deposited By:
Deposited On:
17 Sep 2013 08:08
Refereed?:
Yes
Published?:
Published
Last Modified:
07 Apr 2020 02:35