Solid-state NMR reveals differences in the packing arrangements of peptide aggregates derived from the aortic amyloid polypeptide medin

Davies, Hannah A and Madine, Jillian and Middleton, David A (2012) Solid-state NMR reveals differences in the packing arrangements of peptide aggregates derived from the aortic amyloid polypeptide medin. June 2002. ISSN 1460-4582, 18 (1). pp. 65-72. ISSN 0893-3200

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Abstract

Several polypeptides aggregate into insoluble amyloid fibrils associated with pathologies such as Alzheimer's disease, Parkinson's disease and type 2 diabetes. Understanding the structural and sequential motifs that drive fibrillisation may assist in the discovery and refinement of effective therapies. Here we investigate the effects of three predicted amyloidogenic regions on the structure of aggregates formed by medin, a poorly characterised polypeptide associated with aortic medial amyloidosis. Solid-state NMR is used to compare the dynamics and sheet packing arrangement of the C-terminal region encompassing residues F(43) GSV within full-length medin (Med(1-50) ) and two shorter peptide fragments, Med(30-50) and Med(42-49) , lacking specific sequences predicted to be amyloidogenic.(.) Results show that all three peptides have different aggregate morphologies, and Med(30-50) and Med(1-50) have different sheet packing arrangements and dynamics to Med(42-49) . These results imply that at least two of the three predicted amyloidogenic regions are required for the formation and elongation of medin fibres observed in the disease state.

Item Type:
Journal Article
Journal or Publication Title:
June 2002. ISSN 1460-4582
Additional Information:
Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3200
Subjects:
?? AMINO ACID SEQUENCEAMYLOIDAMYLOIDOSISANTIGENS, SURFACEAORTABIOLOGICAL MARKERSDIABETES MELLITUS, TYPE 2HUMANSMAGNETIC RESONANCE SPECTROSCOPYMICROSCOPY, ELECTRONMILK PROTEINSMOLECULAR SEQUENCE DATANEURODEGENERATIVE DISEASESPEPTIDE FRAGMENTSPROTEIN STRUCTURE ??
ID Code:
66261
Deposited By:
Deposited On:
17 Sep 2013 08:03
Refereed?:
Yes
Published?:
Published
Last Modified:
22 Sep 2023 00:21