Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1

Zhang, Dayi and Zhao, Yun and He, Yi and Wang, Yun and Zhao, Yiyu and Zheng, Yi and Wei, Xia and Zhang, Litong and Li, Yuzhen and Jin, Tao and Wu, Lin and Wang, Hui and Davison, Paul A. and Xu, Junguang and Huang, Wei E. (2012) Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1. ACS Synthetic Biology, 1 (7). pp. 274-283. ISSN 2161-5063

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Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP 1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.

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Journal Article
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ACS Synthetic Biology
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11 Jul 2013 10:35
Last Modified:
17 Sep 2023 01:25