The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies

Jecna, Lucie and Dostalova, Anna and Wilson, Ray and Seblova, Veronika and Chang, Kwang-Poo and Bates, Paul A. and Volf, Petr (2013) The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies. Parasitology, 140 (8). pp. 1026-1032. ISSN 1469-8161

[img]
Preview
PDF (S0031182013000358a)
S0031182013000358a.pdf - Published Version

Download (128kB)

Abstract

SUMMARY Binding of promastigotes to the sand fly midgut epithelium is regarded as an essential part of the Leishmania life cycle in the vector. Among Leishmania surface molecules putatively involved in attachment to the sand fly midgut, two GPI-anchored molecules are the most prominent: lipophosphoglycan (LPG) and promastigote surface protease gp63. In this work, we examined midgut attachment of Leishmania lines mutated in GPI-anchored molecules and compared results from 2 different techniques: in vivo development in sand flies and in vitro competitive binding assays using fluorescently labelled parasites. In combination with previous studies, our data provide additional support for (1) an LPG-independent parasite-binding mechanism of Leishmania major within the midgut of the permissive vector Phlebotomus perniciosus, and provide strong support for (2) the crucial role of L. major LPG in specific vector Phlebotomus papatasi, and (3) a role for Leishmania amazonensis gp63 in Lutzomyia longipalpis midgut binding. Moreover, our results suggest a critical role for GPI-anchored proteins and gp63 in Leishmania mexicana attachment to L. longipalpis midguts, as the wild type (WT) line accounted for over 99% of bound parasites.

Item Type:
Journal Article
Journal or Publication Title:
Parasitology
Additional Information:
http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 140 (8), pp 1026-1032 2013, © 2013 Cambridge University Press.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2405
Subjects:
ID Code:
64817
Deposited By:
Deposited On:
30 May 2013 09:04
Refereed?:
Yes
Published?:
Published
Last Modified:
07 Jul 2020 03:04