An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera:Symphyta).

Graham, Robert Iain and Zahner, Vivane and Lucarotti, Christopher (2008) An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera:Symphyta). Canadian Journal of Microbiology, 54 (9). pp. 758-768. ISSN 0008-4166

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Abstract

Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR–DGGE and PCR–RFLP products revealed a dominance of α- and γ-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).

Item Type: Journal Article
Journal or Publication Title: Canadian Journal of Microbiology
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2400/2404
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 62749
Deposited By: ep_importer_pure
Deposited On: 07 Mar 2013 10:17
Refereed?: Yes
Published?: Published
Last Modified: 01 Jan 2020 08:20
URI: https://eprints.lancs.ac.uk/id/eprint/62749

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