Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector

Dillon, R. J. and Lane, R. P. (1999) Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector. Parasitology, 118 (1). pp. 27-32. ISSN 0031-1820

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Abstract

Binding to the midgut microvillar surface in the sandfly Phlebotomus papatasi is a prerequisite for successful development of Leishmania major within the gut of the vector. This paper describes a method for detecting microvillar-associated proteins which act as ligands for the parasite surface glycoconjugate lipophosphoglycan (LPG). Adhesion of LPG to midgut proteins was visualized by probing midgut extracts with LPG using a Western ligand blotting technique. Procyclic L. major LPG bound to a microvillar polypeptide band of 65 kDa (estimated in the non-reduced state) and bound variably to several lower molecular weight bands, probably degradation products or subunits of the primary binding polypeptides. Specificity of binding was confirmed by co-incubating biotinylated LPG with an LPG-specific mAb which resulted in a great reduction in binding.

Item Type:
Journal Article
Journal or Publication Title:
Parasitology
Additional Information:
http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 118 (1), pp 27-32 1999, © 1999 Cambridge University Press.
Uncontrolled Keywords:
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Subjects:
ID Code:
57952
Deposited By:
Deposited On:
19 Oct 2012 07:58
Refereed?:
Yes
Published?:
Published
Last Modified:
20 Oct 2020 02:11