Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major

Jamjoom, Manal and Ashford, R W and Bates, Paul and Kemp, Stephen J and Noyes, Harry A (2002) Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major. Molecular Ecology Notes, 2 (2). pp. 104-106. ISSN 1471-8286

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Abstract

Thirteen sets of polymerase chain reaction (PCR) primers were designed to amplify microsatellite loci identified in the genome sequence of Leishmania major. Polymorphisms were detected in L. major at all loci. In Leishmania donovani only two of these loci were informative for classification purposes with this data set. The PCR products of all loci from one L. donovani strain were sequenced and it was found that the number of repeats in the microsatellite loci were either substantially reduced with respect to L. major or absent altogether. Consequently it is unlikely to be possible to use the genome sequence of L. major to identify polymorphic microsatellite loci in other Leishmania species.

Item Type:
Journal Article
Journal or Publication Title:
Molecular Ecology Notes
Uncontrolled Keywords:
/dk/atira/pure/core/keywords/biologicalsciences/biomedicalandlifesciences
Subjects:
?? leishmania tropicaleishmania mexicana mapping leishmania infantumbiomedical and life sciencesecologybiochemistrygeneral biochemistry,genetics and molecular biologybiochemistry, genetics and molecular biology(all)qr355 virology ??
ID Code:
56306
Deposited By:
Deposited On:
27 Jul 2012 15:33
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 09:06