A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures

Dluzewski, A R and Ling, I T and Rangachari, K and Bates, P A and Wilson, R J (1984) A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Transactions of The Royal Society of Tropical Medicine and Hygiene, 78 (5). pp. 622-624. ISSN 0035-9203

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Abstract

Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.

Item Type:
Journal Article
Journal or Publication Title:
Transactions of The Royal Society of Tropical Medicine and Hygiene
Uncontrolled Keywords:
/dk/atira/pure/researchoutput/libraryofcongress/qr355
Subjects:
ID Code:
56300
Deposited By:
Deposited On:
27 Jul 2012 15:19
Refereed?:
Yes
Published?:
Published
Last Modified:
18 Feb 2020 06:37