Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesi

Williamson, D H and Wilson, R J and Bates, P A and McCready, S and Perler, F and Qiang, B U (1985) Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesi. Molecular and Biochemical Parasitology, 14 (2). pp. 199-209. ISSN 0166-6851

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Abstract

Restriction analyses and DNA/DNA hybridisation of parasite DNA isolated from monkeys infected with the malarial parasite Plasmodium knowlesi has permitted unambiguous identification of the nuclear DNA of this species. Its (G+C) content, as determined by estimations of buoyant density as well as by direct analysis, is about 38%, essentially indistinguishable from that of its primate laboratory host, and grossly different from that of the major human malaria parasite, P. falciparum, which has a (G+C) content of approx. 19%. In addition, gradient fractionation of total P. knowlesi DNA revealed a minor DNA component (approx. 1% of the total) with a (G+C) content of about 19%. This DNA comprises covalently closed circular molecules which have a contour length about 11.6 microns, carry a small cruciform structure, and are thought to originate in the parasite's mitochondria.

Item Type:
Journal Article
Journal or Publication Title:
Molecular and Biochemical Parasitology
Uncontrolled Keywords:
/dk/atira/pure/researchoutput/libraryofcongress/qr355
Subjects:
ID Code:
56299
Deposited By:
Deposited On:
27 Jul 2012 15:14
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 08:00